Characterization of Agr RNA expression in Drosophila melanogaster

by J. Colleen Karlo

Written in English
Published: Pages: 50 Downloads: 788
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  • Drosophila melanogaster -- Genetics,
  • RNA-protein interactions,
  • Zoology

Edition Notes

Statementby J. Colleen Karlo
The Physical Object
Paginationiii, 50 leaves :
Number of Pages50
ID Numbers
Open LibraryOL15008837M

  Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be important resources for studies of gene structure and function. We describe a general strategy for producing such collections and its implementation, which so far has generated a set of cDNAs corresponding to over 40% of the genes in the fruit fly Drosophila melanogaster. Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. This profilin is required for intercellular cytoplasm transport during Drosophila . Essential RNA-binding protein. May be required for circadian repression of eclosion. Also essential for nurse cell dumping during oogenesis, the process whereby the cytoplasmic contents of nurse cells are transferred to the oocyte late in it's development. In this study, we tested the effect of the stilbene resveratrol on life span, body composition, locomotor activity, stress response, and the expression of genes encoding proteins centrally involved in ageing pathways in the model organism Drosophila melanogaster. Male and female w D. melanogaster were fed diets based on sucrose, corn meal, and yeast.

Roman G, Endo K, Zong L, Davis RL. P[Switch], a system for spatial and temporal control of gene expression in Drosophila melanogaster. Proc Natl Acad Sci U S A. ; – [PMC free article] [Google Scholar] Ruby JG, Jan CH, Bartel DP. Intronic microRNA precursors that bypass Drosha processing. Nature. a; – Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during.   Drosophila melanogaster has served as an indispensable model system for defining the diverse biological roles of miRNAs, their mechanism of action as well as the role of miRNA biogenesis factors. In this chapter we describe some of the assays used for molecular analysis of Drosophila . Characterization of Cas9–Guide RNA Orthologs Jonathan L. Braff, Stephanie J. Yaung, Kevin M. Esvelt, and George M. Church CHAPTER 8 Cas9-Mediated Genome Engineering in Drosophila melanogaster INTRODUCTION Design and Generation of Drosophila Single Guide RNA Expression Constructs Benjamin E. Housden, Yanhui Hu, and Norbert Perrimon.

  Fine mapping of satellite DNA sequences along the Y chromosome of Drosophila melanogaster: relationships between satellite sequences and fertility factors. Genetics Larracuente A.M., Clark A.G. Surprising differences in the variability of Y chromosomes in African and cosmopolitan populations of Drosophila melanogaster.   The third-chromosome group of mutant characters in Drosophila melanogaster. Carnegie Inst. Washington Publ. , – D. et al. Multiplex detection of RNA expression in Drosophila embryos.   These studies have focused on RNA viruses, because all well-established model viruses of Drosophila and all known mosquito-transmitted viruses are RNA viruses. More recently, a next-generation sequencing approach identified small RNAs derived from a novel densovirus, a single-stranded (ss) DNA virus, in wild-caught C. pipiens molestus (25). A full-length lysozyme cDNA from Gryllotalpa orientalis was cloned and sequenced. The deduced amino acid sequence of the lysozyme protein was amino acids in length, with a calculated molecular mass of kDa and an isoelectric point of Sequence motifs, together with alignment and phylog .

Characterization of Agr RNA expression in Drosophila melanogaster by J. Colleen Karlo Download PDF EPUB FB2

Small RNAs ranging in size between 20 and 30 nucleotides are involved in different types of regulation of gene expression including mRNA degradation, translational repression, and chromatin modification. Here we describe the small RNA profile of Drosophila melanogaster as a Cited by: Expression analysis of Drosophila melanogaster microRNAs Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakult ten.

The Antigen 5 related(Agr) gene of Drosophila melanogasterencodes a product that is a member of the CAP family of proteins (Schreiber et al., ). CAP family members have been identified in invertebrate and vertebrate animals, in fungi, and in by: To elucidate the molecular and functional characterization of DSC1, we isolated 20 full-length cDNA clones covering the entire coding region of the DSC1 gene from adults of D.

melanogaster. Sequence analysis revealed that DSC1 transcripts undergo extensive alternative splicing and potential RNA by:   A cloned kb cDNA that hybridizes to genomic clonecontaining genes predominantly expressed in the head of Drosophila melanogaster, was sequencing showed that the cDNA-encoded protein is similar to a family of mammalian proteins, calledwhich activate tyrosine hydroxylase (TyrOHase) and tryptophan hydroxylase (TrpOHase), the two key Cited by:   The corresponding Agr RNA is about 1 kb in length and is present at all stages of development, with highest levels observed in adults.

Agr RNA is transcribed from a single gene that is located within region 12F of the X chromosome. The identification of Agr in Drosophila expands the. Five genes encoding SCP/TAPS proteins have been characterized in the fruit fly Drosophila melanogaster: Antigen 5 -related (Agr) and Antigen 5 -related 2 (Agr 2) and SCP-Related (SCPR)-A, -B, and - C.

Agr and Agr 2 are expressed within the digestive tract. We here describe the cloning and characterization of the functionally active Drosophila melanogaster (Drm) FMRFamide receptor, which we designated as DrmFMRFa-R. The full-length ORF of a D.

melanogaster orphan receptor, CG (Berkeley Drosophila Genome Project), was cloned from genomic DNA. This receptor is distantly related to mammalian thyroid-stimulating hormone. Total RNA Isolation from Drosophila melanogaster (Protocol summary only for purposes of this preview site) Of the methods available for extracting total RNA from Drosophila, monophasic lysis reagents (such as TRIzol) yield consistent and reliable results and are considerably cheaper than other kit-based techniques for RNA the following protocol, up to 50 mg of Drosophila tissue is.

The Genome. THE basic karyotype of Drosophila melanogaster, which can be seen in mitotically active neuroblasts of the larval brain, is comprised by four chromosomes, the X and Y sex chromosomes, two larger autosomal elements, chromosomes 2 and 3, and the small dot fourth chromosome (Metz ; Deng et al.

).The X is also referred to as the First chromosome and. Sawicki R, Singh SP, Mondal AK, Benes H, Zimniak P. Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathione S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon class.

Biochem J. ;(Pt 2)–9. Age-related variations in total nucleic acid levels have been studied in Drosophila melanogaster and several other dipteran species, in some cases with contradictory results. There have been reports of increasing nucleic acid content during the first 5 days of adult life in D.

melanogaster and the mosquito Aedes aegypti (1, 2), while constant levels throughout adult life were reported for the. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and evolution of Michael Ashburner's classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals 5/5(1).

The Ago2 protein is an siRNA-related protein [7, 8] that acts as a core element of RISC in the gene silencing pathways of different insects [9], such as Drosophila melanogaster [10], Bombyx mori [11], Tribolium castaneum [12] and Leptinotarsa decemlineata [13].

In D. melanogaster, Ago2 is essential. has allowed diversification of actin expression and/ or function. The Drosophila melanogaster actin gene family con- sists of six highly conserved genes, dispersed through- out the genome (TOBIN et al. ; FYRBERG et al. ), that exhibit both developmental stage- and tissue-specific expression.

The expression patterns of. we used RNA-Seq and tiling microarrays to sample the Drosophila transcriptome at unprecedented depth throughout development from early embryo to ageing male and female adults.

We report on a high-resolution view of the discovery, structure and dynamic expression of the D. melanogaster transcriptome.

Strategy for characterization of the. The genomic RNA of the gypsy retroelement from Drosophila melanogaster exhibits features similar to other retroviral RNAs because its 5′ untranslated (5′ UTR) region is unusually long ( nucleotides) and potentially highly structured.

Our initial aim was to search for an internal ribosome entry site (IRES) element in the 5′ UTR of the gypsy genomic RNA by using various monocistronic. The spotted-wing Drosophila (Drosophila suzukii) is a widespread invasive pest that causes severe economic damage to fruit crops.

The early development of D. suzukii is similar to that of other Drosophilids, but the roles of individual genes must be confirmed experimentally.

Cellularization genes coordinate the onset of cell division as soon as the invagination of membranes starts around the. Dastarcus helophoroides, which has a relatively longer lifespan compared to other insects, is one of the most effective natural enemies of many large-body long-horned beetles.

Methuselah (Mth) is associated with the lifespan, stress resistance, and reproduction in Drosophila melanogaster, but Mth is not present in non-drosophiline insects. A number of methuselah-like genes (mth-likes, mthls. - Methods for the study of innate immunity in Drosophila melanogaster.

Troha K,Buchon N pmid: ; - Systemic bacterial infection and immune defense phenotypes in Drosophila melanogaster. Khalil S,Jacobson E,Chambers MC,Lazzaro BP pmid:   To better understand the role(s) of hnRNP proteins in the process of mRNA formation, we have identified and characterized the major nuclear proteins that interact with hnRNAs in Drosophila melanogaster.

cDNA clones of several D. melanogaster hnRNP proteins have been isolated and sequenced, and the genes encoding these proteins have been mapped cytologically on polytene.

Animal microRNAs (miRNA) are implicated in the control of nearly all cellular functions. Due to high sequence redundancy within the miRNA gene pool, loss of most of these to bp long RNAs individually does not cause a phenotype.

Thus, only very few miRNAs have been associated with clear functional roles. We constructed a transgenic UAS-miRNA library in Drosophila melanogaster. Title: Purification and Characterization of Recombinant Lipid Storage Protein-2 from Drosophila melanogaster VOLUME: 15 ISSUE: 9 Author(s):Estela L.

Arrese, Laticia Rivera, Masakazu Hamada and Jose L. Soulages Affiliation Noble Research Center, Department of Biochemistry and Molecular Biology, Oklahoma State University Stillwater, OK,USA.

Gene elav of Drosophila melanogaster: A prototype for neuronal‐specific RNA binding protein gene family that is conserved in flies and humans Kwok‐Ming Yao Department of Biology and Center for Complex Systems, Brandeis University, Waltham, Massachusetts   In Drosophila, transposons and repeated sequences, including P-element, Stellate, I-element, and gypsy, are repressed by a trans-silencing mechanism termed “cosuppression” that targets any transposons containing homologous sequences to the “trigger” transcripts by small interfering RNA (siRNA; refs.

33–35). Recent comparative studies have indicated distinct expression profiles of short, non-coding microRNAs (miRNAs) in various types of cancer, including oral squamous cell carcinoma (OSCC). In this study, we employed a hybrid approach using Drosophila melanogaster as well as OSCC cell lines to validate putative targets of oral cancer-related miRNAs both in vivo and in vitro.

Figure Distribution of β-galactosidase expression in DJ across Age and in Tissues 45 Figure Genomic Region of DJ 46 CHAPTER 2 Figure Luc RNA as a Spike Molecule in Different Species 68 Figure RT Efficiency of Various Initial Concentrations of Luc RNA 69 Figure The Influence of Background RNA on the RT Efficiency of.

Gomisin N (GN) is lignin derived from Schisandra chinensis that has been reported to exhibit hepato-protective, anti-cancer, and anti-inflammatory effects.

However, its role in whole-body energetic homeostasis remains unclear. In this study, we employed Drosophila melanogaster as a diet-induced obese model to elucidate the effects of GN on lipid and glucose metabolism by measuring climbing.

Caizzi R, Ritossa F. The enzyme glutamine synthetase I of Drosophila melanogaster is associated with a modified RNA.

Biochem. Genet. Campbell HD, Webb GC, Young IG. A human homologue of the Drosophila melanogaster sluggish-A (proline oxidase) gene maps to 22q, and is a candidate gene for type-I hyperprolinaemia. Hum. subunit of the a polymerase from Drosophila melanogas-ter.

The protein shows high similarity to the kDa subunits from other species. Comparative expression analysis for the transcript, protein and enzymic activity suggests that control occurs mainly at the level of transcription.

In situ analyses of the RNA suggest that. vg is expressed in a subset of cells in the ventral nerve cord including interneurons descending from neuroblasts NB, NB, and NB vg-positive cells are first detected at embryonic stage 12 ( cells).By st each thoracic segment contains vg-positive cells that divide into six clusters, three located in a dorsal plane and three located in a ventral plane.Characterization of the flamenco Region of the Drosophila melanogaster Genome Vale´rie Robert, Nicole Prud’homme, Alexander Kim,1 Alain Bucheton and Alain Pe´lisson CGM/CNRS, Gif-sur-Yvette, France and IGH/CNRS, Montpellier, France Manuscript received Aug Accepted for publication March 9, ABSTRACT.Ribonucleases belonging to the RNase T2 family are enzymes associated with the secretory pathway that are almost absolutely conserved in all eukaryotes.

Studies in plants and vertebrates suggest they have an important housekeeping function in rRNA recycling. However, little is known about this family of enzymes in protostomes. We characterized RNase X25, the only RNase T2 enzyme in Drosophila.